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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX114914 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114914, RRID:AB_10623571
- Product name
- Aldolase B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) C: JurKat D: Raji 10% SDS PAGE GTX114914 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse liver 10% SDS PAGE GTX114914 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aldolase B antibody detects ALDOB protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract10 % SDS-PAGEAldolase B antibody (GTX114914) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX114914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using Aldolase B(GTX114914) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. Aldolase B antibody (GTX114914) dilution: 1:500.
