18065-1-AP

antibody from Invitrogen Antibodies

Targeting: ALDOB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18065-1-AP

Provider

Invitrogen Antibodies

Product name

ALDOB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAHRFPALT QEQKKELSEI AQSIVANGKG ILAADESVGT MGNRLQRIKV ENTEENRRQF REILFSVDSS INQSIGGVIL FHETLYQKDS QGKLFRNILK EKGIVVGIKL DQGGAPLAGT NKETTIQGLD GLSERCAQYK KDGVDFGKWR AVLRIADQCP SSLPIQENAN ALARYASICQ QNGLVPIVEP EVIPDGDHDL EHCQYVTEKV LAAVYKALND HHVYLEGTLL KPNMVTAGHA CTKKYTPEQV AMATVTALHR TVPAAVPGIC FLSGGMSEED ATLNLNAINL CPLPKPWKLS FSGVARLQTR RQPRRLL (1-316 aa encoded by BC029399)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.41 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP (ALDOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ALDOB antibody 18065-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 18065-1-AP (ALDOB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 18065-1-AP (ALDOB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 18065-1-AP) (ALDOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ALDOB (IP:18065-1-AP, 4 ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:500) with mouse brain tissue lysate 2400 ug.