Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-87494 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87494, RRID:AB_11029448
- Product name
- Rabbit Polyclonal Aldolase B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Aldolase B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The human liver-specific proteome defined by transcriptomics and antibody-based profiling.
Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution.
Kampf C, Mardinoglu A, Fagerberg L, Hallström BM, Edlund K, Lundberg E, Pontén F, Nielsen J, Uhlen M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jul;28(7):2901-14
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jul;28(7):2901-14
Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution.
Merkulova M, Hurtado-Lorenzo A, Hosokawa H, Zhuang Z, Brown D, Ausiello DA, Marshansky V
American journal of physiology. Cell physiology 2011 Jun;300(6):C1442-55
American journal of physiology. Cell physiology 2011 Jun;300(6):C1442-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldolase B Antibody [NBP1-87494] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431 Lane 5: Human liver tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldolase B Antibody [NBP1-87494] - Analysis in human kidney tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining of human kidney shows moderate cytoplasmic positivity in cells in proximal tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldolase B Antibody [NBP1-87494] - Staining in human kidney and tonsil tissues . Corresponding ALDOB RNA-seq data are presented for the same tissues.