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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00005972-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005972-M01, RRID:AB_425655
- Product name
- REN monoclonal antibody (M01), clone 2H2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant REN.
- Antigen sequence
LPTDTTTFKRIFLKRMPSIRESLKERGVDMARLGP
EWSQPMKRLTLGNTTSSVILTNYMDTQYYGEIGIG
TPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHK
LFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDII
TVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGF
IEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSE
NSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGVWQ
IQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTS
SIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHL
GGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPP
PTGPTWALGATFIRKFYTEFDRRNNRIGFALAR- Isotype
- IgG
- Antibody clone number
- 2H2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references HIV-induced kidney cell injury: role of ROS-induced downregulated vitamin D receptor.
Salhan D, Husain M, Subrati A, Goyal R, Singh T, Rai P, Malhotra A, Singhal PC
American journal of physiology. Renal physiology 2012 Aug 15;303(4):F503-14
American journal of physiology. Renal physiology 2012 Aug 15;303(4):F503-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- REN monoclonal antibody (M01), clone 2H2 Western Blot analysis of REN expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged REN is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
