MA5-29523

antibody from Invitrogen Antibodies

Targeting: REN

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29523

Provider

Invitrogen Antibodies

Product name

Renin Recombinant Rabbit Monoclonal Antibody (009)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

9

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Renin in Lane A: K562 Whole Cell Lysate (30 µg). Samples were probed using a Renin Monoclonal Antibody (Product # MA5-29523) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:45 kDa. Observed band size:57 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human Renin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Renin Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29523, 1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human Renin IN in human kidney with Renin Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29523, 1:1,000 dilution, formalin-fixed paraffin embedded sections).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Human Renin expression on Jurkat cells. The cells were treated according to manufacturer’s manual, stained with Renin Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29523), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.