TA324617

antibody from OriGene

Targeting: RBPJ CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBPJK, RBPSUH, SUH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324617

Provider

OriGene

Product name

Rabbit polyclonal RBPJ Antibody (N-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal RBPJ Antibody (N-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

RBPJ

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

RBPJ Antibody (N-term) (Cat. #TA324617) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of A2058 cell stained with RBPJ Antibody (N-term)(Cat#TA324617). A2058 cells were incubated with RBPJ primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . RBPJ immunoreactivity is localized to nucleus significantly.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

RBPJ Antibody (N-term) (TA324617)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Validation comment

IHC