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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF792 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IGF-II/IGF2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IGF-II/IGF2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IGF-II/IGF2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P09535- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A CCR2+ myeloid cell niche required for pancreatic β cell growth.
The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
Role of IGF signaling in olfactory sensory map formation and axon guidance.
GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion.
Hypoxia-independent overexpression of hypoxia-inducible factor 1alpha as an early change in mouse hepatocarcinogenesis.
Mussar K, Pardike S, Hohl TM, Hardiman G, Cirulli V, Crisa L
JCI insight 2017 Aug 3;2(15)
JCI insight 2017 Aug 3;2(15)
The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
Ostrovsky O, Ahmed NT, Argon Y
Molecular biology of the cell 2009 Mar;20(6):1855-64
Molecular biology of the cell 2009 Mar;20(6):1855-64
Role of IGF signaling in olfactory sensory map formation and axon guidance.
Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
Neuron 2008 Mar 27;57(6):847-57
Neuron 2008 Mar 27;57(6):847-57
GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion.
Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y
Molecular biology of the cell 2007 Oct;18(10):3764-75
Molecular biology of the cell 2007 Oct;18(10):3764-75
Hypoxia-independent overexpression of hypoxia-inducible factor 1alpha as an early change in mouse hepatocarcinogenesis.
Tanaka H, Yamamoto M, Hashimoto N, Miyakoshi M, Tamakawa S, Yoshie M, Tokusashi Y, Yokoyama K, Yaginuma Y, Ogawa K
Cancer research 2006 Dec 1;66(23):11263-70
Cancer research 2006 Dec 1;66(23):11263-70
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGF-II/IGF2 in Mouse Embryo. IGF-II/IGF2 was detected in immersion fixed frozen sections of mouse embryo (E13-17) using Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IGF-II/IGF2 and Neutralization by Mouse IGF-II/IGF2 Antibody. Recombinant Mouse IGF-II/IGF2 (Catalog # 792-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-II/IGF2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792). The ND50 is typically 0.5-2.5 µg/mL.
