600-401-679

antibody from Rockland Immunochemicals, Inc.

Targeting: RAD52

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-679

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-679, RRID:AB_2300816

Product name

Anti-Human RAD52 (RABBIT) Antibody - 600-401-679

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Formation of polarized contractile interfaces by self-organized Toll-8/Cirl GPCR asymmetry.

Lavalou J, Mao Q, Harmansa S, Kerridge S, Lellouch AC, Philippe JM, Audebert S, Camoin L, Lecuit T
Developmental cell 2021 Jun 7;56(11):1574-1588.e7

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis is shown using Rockland's Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B).   ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected.  Peptide competition (panel A) blocks the specific staining of this band.  The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:5,000 for 45 min.  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by

Validation comment

Western Blot