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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX111627 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111627, RRID:AB_11175861
- Product name
- Calreticulin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.
Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.
EDEM2 and OS-9 are required for ER-associated degradation of non-glycosylated sonic hedgehog.
Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Kuo SM, Chen CJ, Chang SC, Liu TJ, Chen YH, Huang SY, Shih SR
mBio 2017 Jun 13;8(3)
mBio 2017 Jun 13;8(3)
Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.
Huang JH, Ku WC, Chen YC, Chang YL, Chu CY
Scientific reports 2017 Feb 20;7:42853
Scientific reports 2017 Feb 20;7:42853
Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF
Disease models & mechanisms 2016 Aug 1;9(8):885-97
Disease models & mechanisms 2016 Aug 1;9(8):885-97
Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.
Hulleman JD, Kelly JW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Feb;29(2):565-75
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Feb;29(2):565-75
EDEM2 and OS-9 are required for ER-associated degradation of non-glycosylated sonic hedgehog.
Tang HY, Huang CH, Zhuang YH, Christianson JC, Chen X
PloS one 2014;9(6):e92164
PloS one 2014;9(6):e92164
Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.
Law CH, Li JM, Chou HC, Chen YH, Chan HL
Toxicology 2013 Jan 7;303:54-71
Toxicology 2013 Jan 7;303:54-71
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D:HepG2 10% SDS PAGE GTX111627 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calreticulin antibody detects Calreticulin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Calreticulin antibody (GTX111627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calreticulin antibody detects Calreticulin protein at endoplasmic reticulum by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calreticulin protein stained by Calreticulin antibody (GTX111627) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Calreticulin(GTX111627) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse muscle, using Calreticulin(GTX111627) antibody at 1:500 dilution.
