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LearnPA5-53255
antibody from Invitrogen Antibodies
Targeting: DENND4C
bA513M16.3, C9orf55, C9orf55B, FLJ20686, RAB10GEF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-53255 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DENND4C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PSGIFDVNSR KSSTGSISNV LFSTQDPVED AVFGEATNLK KNGDRGEKRQ KHFPERSCSF SSESRAGMLL KKSSLDSNSS EMAIMMGADA KILTAALTCP KTSLLHIART HSFENVSCHL PDSRTCMSES TW
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DENND4C in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DENND4C Polyclonal Antibody (Product # PA5-53255).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DENND4C in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DENND4C Polyclonal Antibody (Product # PA5-53255).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DENND4C in human cell line A-431 shows positivity in cytoplasm, the Golgi apparatus & vesicles. Samples were probed using a DENND4C Polyclonal Antibody (Product # PA5-53255).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND4C in human duodenum using DENND4C Polyclonal Antibody (Product # PA5-53255) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND4C in human skin using DENND4C Polyclonal Antibody (Product # PA5-53255) shows moderate cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND4C in human fallopian tube using DENND4C Polyclonal Antibody (Product # PA5-53255) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DENND4C in human skeletal muscle using DENND4C Polyclonal Antibody (Product # PA5-53255) shows no positivity in myocytes as expected.
