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LearnLS-C288560
antibody from LSBio
Targeting: NIPBL
DKFZp434L1319, FLJ11203, FLJ12597, FLJ13354, FLJ13648, IDN3, Scc2
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- LS-C288560 - Provider product page
- Provider
- LSBio
- Product name
- Anti-IDN3 / NIPBL Antibody (aa1025-1075) LS-C288560
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Bovine, Canine, Horse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Store at 2-8°C for up to 1 year.
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Supportive validation
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- LSBio (provider)
- Main image
- Experimental details
- Detection of Human NIPBL by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NIPBL antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NIPBL was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
