Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00078990-M14 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00078990-M14, RRID:AB_2157000
- Product name
- Mouse Monoclonal Otubain-2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. OTUB2 - OTU domain, ubiquitin aldehyde binding 2 (2E7)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Regulation of Gli2 stability by deubiquitinase OTUB2.
Li XY, Mao XF, Tang XQ, Han QQ, Jiang LX, Qiu YM, Dai J, Wang YX
Biochemical and biophysical research communications 2018 Oct 20;505(1):113-118
Biochemical and biophysical research communications 2018 Oct 20;505(1):113-118
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Otubain-2 Antibody (2E7) [H00078990-M14] - Detection limit for recombinant GST tagged OTUB2 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14] - Analysis of OTUB2 transfected lysate using anti-OTUB2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OTUB2 rabbit polyclonal antibody.