MA5-13871

antibody from Invitrogen Antibodies

Targeting: MVP LRP, VAULT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13871

Provider

Invitrogen Antibodies

Product name

MVP Monoclonal Antibody (1032)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-13871 targets Major Vault Protein in FACS, GS, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

Antibody clone number

1032

Concentration

0.2 mg/mL

References

Submitted references

Proteome Profiling of RNF213 Depleted Cells Reveals Nitric Oxide Regulator DDAH1 Antilisterial Activity.

Martina L, Asselman C, Thery F, Boucher K, Delhaye L, Maia TM, Dermaut B, Eyckerman S, Impens F
Frontiers in cellular and infection microbiology 2021;11:735416

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MVP expression in the prediction of clinical outcome of locally advanced oral squamous cell carcinoma patients treated with radiotherapy.

Henríquez-Hernández LA, Moreno M, Rey A, Lloret M, Lara PC
Radiation oncology (London, England) 2012 Aug 29;7:147

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Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.

Guan J, Chen XP, Zhu H, Luo SF, Cao B, Ding L
World journal of gastroenterology 2004 Dec 1;10(23):3522-7

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Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.

Guan J, Chen XP, Zhu H, Luo SF, Cao B, Ding L
World journal of gastroenterology 2004 Dec 1;10(23):3522-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Major Vault Protein using Major Vault Protein Monoclonal Antibody (Product # MA5-13871) on SKBR3 Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), T98G (Lane 2), A549 (Lane3), PANC-1(Lane 4), HeLa (Lane 5) and HeLa treated with Interferon gamma (250IU/mL) for 48 hours (Lane 6). The blot was probed with MVP Monoclonal Antibody (Product # MA5-13871, 0.2 mg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to MVP was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human breast carcinoma stained with LRP/MVP antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 RNF213 knockdown downregulates DDAH1 and CYR61 at the transcript level. HeLa cells were treated with IFN-beta in combination with knockdown of RNF213 as described in Figure 1 . (A) Immunoblotting on the actual samples used for proteome analysis confirmed efficient knockdown of RNF213 and interferon-induced upregulation of free and conjugated ISG15. Immunoblots against DDAH1, CYR61 and MVP validated downregulation of DDAH1 and CYR61, and upregulation of MVP upon knockdown of RNF213. Tubulin was used as loading control. Densitometry and statistical analysis on two more biological repeats confirmed significant up- or downregulation of the immunoblotting bands ( Supplementary Figure 3 ). (B) Real time qPCR upon knockdown of RNF213 showed significant upregulation of MVP in the presence of IFN-beta and significant downregulation of CYR61 and DDAH1 transcripts in the presence and absence of IFN-beta. Expression was normalized to housekeeping genes and shown relative to control (siScramble) populations set to 1. Data represents 3 biological replicates with 4 technical replicates per condition. (AVG +- SEM, two-tailed unpaired t-test (n = 3), *p < 0.05 and ***p < 0.001).