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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX105193 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105193, RRID:AB_1951019
- Product name
- NR0B1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: HeLa S3 (GTX14654)7.5% SDS PAGEGTX105193 diluted at 1:500
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NR0B1 antibody detects NR0B1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NR0B1 protein stained by NR0B1 antibody (GTX105193) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 ?g of NRB1 antibody (GTX105193) 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody (GTX105193) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NR0B1(GTX105193) antibody at 1:100 dilution.
