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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NB120-20600-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Glycerol Kinase Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination.
- Reactivity
- Bacteria
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glycerol Kinase Antibody [Biotin] [NB120-20600] - Detection of purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glycerol Kinase Antibody [Biotin] [NB120-20600] - Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.
