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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 13360-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glycerokinase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAASKKAVL GPLVGAVDQG TSSTRFLVFN SKTAELLSHH QVEIKQEFPR EGWVEQDPKE ILHSVYECIE KTCEKLGQLN IDISNIKAIG VSNQRETTVV WDKITGEPLY NAVVWLDLRT QSTVESLSKR IPGNNNFVKS KTGLPLSTYF SAVKLRWLLD NVRKVQKAVE EKRALFGTID SWLIWSLTGG VNGGVHCTDV TNASRTMLFN IHSLEWDKQL CEFFGIPMEI LPNVRSSSEI YGLMKAGALE GVPISGCLGD QSAALVGQMC FQIGQAKNTY GTGCFLLCNT GHKCVFSDHG LLTTVAYKLG RDKPVYYALE GSVAIAGAVI RWLRDNLGII KTSEEIEKLA KEVGTSYG (1-357 aa encoded by BC037549)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 13360-1-AP (Glycerokinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 13360-1-AP (Glycerokinase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Glycerokinase (IP:13360-1-AP, 4ug; Detection:13360-1-AP 1:1000) with HepG2 cells lysate 4000ug.
