PAB22081

antibody from Abnova Corporation

Targeting: NUP153 HNUP153

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22081

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22081, RRID:AB_10983999

Product name

NUP153 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NUP153.

Antigen sequence

GQNREQRESGFSYPNFSLPAANGLSSGVGGGGGKM
RRERTRFVASKPLEEEEMEVPVLPKISLPITSSSL
PTFNFSSPEITTSSPSPINSSQALTNKVQMTSP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NUP153 polyclonal antibody (Cat # PAB22081) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with NUP153 polyclonal antibody (Cat # PAB22081) at 1-4 ug/mL dilution shows positivity in nuclear membrane.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with NUP153 polyclonal antibody (Cat # PAB22081) shows strong nuclear and nuclear membranous positivity at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)