Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG54735 - Provider product page
- Provider
- Arigo
- Product name
- anti-Calpain1 antibody
- Antibody type
- Monoclonal
- Antigen
- KLH-conjugated synthetic peptide from Human CAPN1 protein (NP_001185797.1).
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 1376CT809.24.85
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) MCF-7, 2) HeLa, 3) U87-MG, and 4) HCT116 lysate stained with ARG54735 anti-Calpain1 antibody at 1:500 dilution.Data provided by customer.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of A431, A549, MCF-7 and U-251 MG cell line lysates (from left to right) stained with ARG54735 anti-Calpain1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human kidney section stained with ARG54735 anti-Calpain1 antibody at 1:25 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG54735 anti-Calpain1 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
