H00000823-D01P

antibody from Abnova Corporation

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000823-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000823-D01P, RRID:AB_1673114

Product name

CAPN1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CAPN1 protein.

Antigen sequence

MSEEIITPVYCTGVSAQVQKQRARELGLGRHENAI
KYLGQDYEQLRVRCLQSGTLFRDEAFPPVPQSLGY
KDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATRT
DICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQS
FQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKL
VFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGST
SEGFEDFTGGVTEWYELRKAPSDLYQIILKALERG
SLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAK
QVNYRGQVVSLIRMRNPWGEVEWTGAWSDSSSEWN
NVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEI
CNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCR
NYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFV
LALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQ
PAVHLKRDFFLANASRARSEQFINLREVSTRFRLP
PGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELD
DQIQANLPDEQVLSEEEIDENFKALFRQLAGEDME
ISVKELRTILNRIISKHKDLRTKGFSLESCRSMVN
LMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLD
KSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSE
PDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVV
TFDLFKWLQLTMFA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CAPN1 expression in transfected 293T cell line (H00000823-T02) by CAPN1 MaxPab polyclonal antibody.Lane 1: CAPN1 transfected lysate(81.90 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to CAPN1 on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CAPN1 and F2R. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal 1:1200 and anti-F2R mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)