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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-27681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mu-Calpain Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.97 mg/mL
Submitted references Regulation of TET protein stability by calpains.
Wang Y, Zhang Y
Cell reports 2014 Jan 30;6(2):278-84
Cell reports 2014 Jan 30;6(2):278-84
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of calpain 1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a calpain 1 polyclonal antibody (Product # PA5-27681) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Mu-Calpain was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Mu-Calpain Polyclonal Antibody (Product # PA5-27681) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Mu-Calpain was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Mu-Calpain Polyclonal Antibody (Product # PA5-27681) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of calpain 1 in methanol-fixed A431 cells using a calpain 1 polyclonal antibody (Product # PA5-27681) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mu-Calpain Polyclonal Antibody detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Mu-Calpain Polyclonal Antibody (Product # PA5-27681) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1 (Product # PA5-27681) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 µg of Mu-Calpain Polyclonal Antibody (Product # PA5-27681). Western blot analysis was performed using Mu-Calpain Polyclonal Antibody (Product # PA5-27681).
