PA5-27681

antibody from Invitrogen Antibodies

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27681

Provider

Invitrogen Antibodies

Product name

Mu-Calpain Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (88%), Rat (88%), Pig (95%), Sheep (94%), Rhesus Monkey (98%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.97 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Regulation of TET protein stability by calpains.

Wang Y, Zhang Y
Cell reports 2014 Jan 30;6(2):278-84

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of calpain 1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a calpain 1 polyclonal antibody (Product # PA5-27681) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Mu-Calpain was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Mu-Calpain Polyclonal Antibody (Product # PA5-27681) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Mu-Calpain was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Mu-Calpain Polyclonal Antibody (Product # PA5-27681) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of calpain 1 in methanol-fixed A431 cells using a calpain 1 polyclonal antibody (Product # PA5-27681) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mu-Calpain Polyclonal Antibody detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Mu-Calpain Polyclonal Antibody (Product # PA5-27681) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1 (Product # PA5-27681) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 µg of Mu-Calpain Polyclonal Antibody (Product # PA5-27681). Western blot analysis was performed using Mu-Calpain Polyclonal Antibody (Product # PA5-27681).