AP14020a

antibody from Abgent

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP14020a

Provider

Abgent

Proper citation

Abgent Cat#AP14020a, RRID:AB_10891612

Product name

BIRC3 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

BIRC3 Antibody (N-term) (Cat. #AP14020a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the BIRC3 antibody detected the BIRC3 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of BIRC3 (arrow) using rabbit polyclonal BIRC3 Antibody (N-term) (Cat. #AP14020a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BIRC3 gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of BIRC3 Antibody (N-term)(Cat#AP14020a) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

BIRC3 Antibody (N-term) (Cat. #AP14020a)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BIRC3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50