NBP1-90132

antibody from Novus Biologicals

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-90132

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-90132, RRID:AB_11017397

Product name

Rabbit Polyclonal cIAP-2/HIAP-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human cIAP-2/HIAP-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Phase I trial of induction histone deacetylase and proteasome inhibition followed by surgery in non-small-cell lung cancer.

Jones DR, Moskaluk CA, Gillenwater HH, Petroni GR, Burks SG, Philips J, Rehm PK, Olazagasti J, Kozower BD, Bao Y
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2012 Nov;7(11):1683-90

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Zoledronic acid directly suppresses cell proliferation and induces apoptosis in highly tumorigenic prostate and breast cancers.

Almubarak H, Jones A, Chaisuparat R, Zhang M, Meiller TF, Scheper MA
Journal of carcinogenesis 2011 Jan 15;10:2

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Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.

Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Analysis in human cell line Daudi.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Analysis in control (vector only transfected HEK293T lysate) and BIRC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Mouse neonatal cardiac myocytes in under normal and hypoxic conidtions. Wb image submitted by verified customer review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human tonsil shows nuclear positivity in reaction center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human cerebral cortex shows positivity in endothelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse cerebellum shows strong immunoreactivity in Purkinje cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse olfactory bulb shows cytoplasmic staining in mitral and external plexiform cell layers.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse piriform cortex shows cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse thalamus shows neuronal staining in paraventricular nucleus.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse hippocampus shows cytoplasmic positivity in a subset of neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse brain shows strong positivity in the mitral cell layer of the olfactory bulb.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of mouse cerebellum shows strong positivity in Purkinje cells in the cerebellum.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human tonsil shows moderate to strong nuclear and cytoplasmic positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining in human tonsil and skeletal muscle tissues using NBP1-90132 antibody. Corresponding BIRC3 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.