AF8171

antibody from R&D Systems

Targeting: BIRC3 API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF8171

Provider

R&D Systems

Product name

Human cIAP-2/HIAP-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human cIAP-2/HIAP-1 in Western blots. In Western blots, less than 1% cross-reactivity with cIAP-1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

U45878


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Absence of Cytosolic 2-Cys Prx Subtypes I and II Exacerbates TNF-α-Induced Apoptosis via Different Routes.

Lee S, Lee JY, Lee EW, Park S, Kang DH, Min C, Lee DJ, Kang D, Song J, Kwon J, Kang SW
Cell reports 2019 Feb 19;26(8):2194-2211.e6

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ASTX660, a Novel Non-peptidomimetic Antagonist of cIAP1/2 and XIAP, Potently Induces TNFα-Dependent Apoptosis in Cancer Cell Lines and Inhibits Tumor Growth.

Ward GA, Lewis EJ, Ahn JS, Johnson CN, Lyons JF, Martins V, Munck JM, Rich SJ, Smyth T, Thompson NT, Williams PA, Wilsher NE, Wallis NG, Chessari G
Molecular cancer therapeutics 2018 Jul;17(7):1381-1391

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Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma.

Liapis V, Zysk A, DeNichilo M, Zinonos I, Hay S, Panagopoulos V, Shoubridge A, Difelice C, Ponomarev V, Ingman W, Atkins GJ, Findlay DM, Zannettino ACW, Evdokiou A
Cancer medicine 2017 Sep;6(9):2164-2176

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Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies.

Mędra A, Witkowska M, Majchrzak A, Cebula-Obrzut B, Bonner MY, Robak T, Arbiser JL, Smolewski P
Molecules (Basel, Switzerland) 2016 Jul 30;21(8)

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USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics.

Lee EW, Seong D, Seo J, Jeong M, Lee HK, Song J
Cell death and differentiation 2015 Sep;22(9):1463-76

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Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).

Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins GJ, Findlay DM, Zannettino AC, Evdokiou A
Anticancer research 2014 Dec;34(12):7007-20

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The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.

Krepler C, Chunduru SK, Halloran MB, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman EM, Benetatos C, Nathanson KL, Amaravadi RK, Pehamberger H, McKinlay M, Herlyn M
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Apr 1;19(7):1784-94

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Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.

Engesæter B, Engebraaten O, Flørenes VA, Mælandsmo GM
PloS one 2012;7(9):e45492

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cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.

Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
Cancer research 2005 Jan 1;65(1):210-8

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Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.

McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
Experimental cell research 2005 Jan 1;302(1):69-82

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human cIAP-2/HIAP-1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP-2/HIAP-1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human cIAP-2/HIAP-1 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-2/HIAP-1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

cIAP-2/HIAP-1 in Human Colon. cIAP-2/HIAP-1 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human cIAP-2/HIAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.