16932-1-AP

antibody from Invitrogen Antibodies

Targeting: AP1B1 ADTB1, AP105A, BAM22, CLAPB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16932-1-AP

Provider

Invitrogen Antibodies

Product name

AP1B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NFVAPPTAA VPANLGAPIG SGLSDLFDLT SGVGTLSGSY VAPKAVGSIS MDLQLTNKAL QVMTDFAIQF NRNSFGLAPA APLQVHAPLS PNQTVEISLP LSTVGSVMKM EPLNNLQVAV KNNIDVFYFS TLYPLHILFV EDGKMDRQMF LATWKDIPNE NEAQFQIRDC PLNAEAASSK LQSSNIFTVA KRNVEGQDML YQSLKLTNGI WVLAELRIQP GNPSCTLSLK CRAPEVSQHV YQAYETILKN (671-919 aa encoded by BC046242)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16932-1-AP (AP1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using AP1B1 antibody 16932-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AP1B1 (IP:16932-1-AP, 4 ug; Detection:16932-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AP1B1 (IP:16932-1-AP, 4ug; Detection:16932-1-AP 1:500) with HEK-293 cells lysate 2000ug.