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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ7145 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ARHGAP45 Antibody / Rho GTPase-activating protein 45
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ARHGAP45 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Raji and 3) A549 cell lysate with ARHGAP45 antibody. Predicted molecular weight ~125 kDa but can be observed at higher molecular weights.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human B lymphocytic tumor tissue with ARHGAP45 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human SiHa cells with ARHGAP45 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ARHGAP45 antibody.
