Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2464 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2464, RRID:AB_1581542
- Product name
- TXNL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TXNL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cloning and expression of a cDNA encoding a human thioredoxin-like protein.
Miranda-Vizuete A, Gustafsson JA, Spyrou G
Biochemical and biophysical research communications 1998 Feb 4;243(1):284-8
Biochemical and biophysical research communications 1998 Feb 4;243(1):284-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The TXNL1 polyclonal antibody (Cat # PAB2464) is used in Western blot to detect TXNL1 in HL-60 cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer.TXNL1 polyclonal antibody (Cat # PAB2464) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.The results correspond precisely to TXNL1 mRNA expression shown with in situ hybridization (JimAcnez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # P0347).The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen.Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.
- Validation comment
- Immunohistochemistry (Frozen sections)