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LearnPAB3289
antibody from Abnova Corporation
Targeting: NEK9
DKFZp434D0935, MGC16714, Nek8, NERCC, NERCC1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3289 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3289, RRID:AB_1577660
- Product name
- NEK9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of NEK9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Nek9, a novel FACT-associated protein, modulates interphase progression.
A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.
Tan BC, Lee SC
The Journal of biological chemistry 2004 Mar 5;279(10):9321-30
The Journal of biological chemistry 2004 Mar 5;279(10):9321-30
A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J
The Journal of biological chemistry 2003 Sep 12;278(37):34897-909
The Journal of biological chemistry 2003 Sep 12;278(37):34897-909
Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.
Roig J, Mikhailov A, Belham C, Avruch J
Genes & development 2002 Jul 1;16(13):1640-58
Genes & development 2002 Jul 1;16(13):1640-58
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The NEK9 polyclonal antibody (Cat # PAB3289) is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK9 polyclonal antibody (Cat # PAB3289) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
