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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX114374 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114374, RRID:AB_10619460
- Product name
- ECH1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX114374 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE ECH1 antibody GTX114374 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody (GTX114374) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ECH1(GTX114374) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody (GTX114374) dilution: 1:500.
