A01700-1

antibody from Boster Biological Technology

Targeting: BIRC2 API1, c-IAP1, cIAP1, hiap-2, MIHB, RNF48

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01700-1

Provider

Boster Biological Technology

Product name

Anti-cIAP1/BIRC2 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CIAP1/BIRC2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. CIAP1/BIRC2 information: Subcellular Localization: Cytoplasm. Nucleus. Agents that induce either the extrinsic or intrinsic apoptotic pathways promote its redistribution from the nuclear compartment to the cytoplasmic compartment. Associated with the midbody in telophase cells, and found diffusely in the nucleus of interphase cells; Tissue Specificity: Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of cIAP1 using anti-cIAP1 antibody (A01700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human COLO-320 whole cell lysates,Lane 5: human U-87MG whole cell lysates,Lane 6: human A549 whole cell lysates,Lane 7: rat thymus tissue lysates,Lane 8: mouse thymus tissue lysates,Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP1 antigen affinity purified polyclonal antibody (Catalog # A01700-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP1 at approximately 70KD. The expected band size for cIAP1 is at 70KD.

Additional image