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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1001 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Frizzled-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q61086- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SNX27 Deletion Causes Hydrocephalus by Impairing Ependymal Cell Differentiation and Ciliogenesis.
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Wnt5a regulates midbrain dopaminergic axon growth and guidance.
Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
Wang X, Zhou Y, Wang J, Tseng IC, Huang T, Zhao Y, Zheng Q, Gao Y, Luo H, Zhang X, Bu G, Hong W, Xu H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Dec 14;36(50):12586-12597
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Dec 14;36(50):12586-12597
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Shi Y, Shu J, Gelman BB, Lisinicchia JG, Tang SJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
Wnt5a regulates midbrain dopaminergic axon growth and guidance.
Blakely BD, Bye CR, Fernando CV, Horne MK, Macheda ML, Stacker SA, Arenas E, Parish CL
PloS one 2011 Mar 31;6(3):e18373
PloS one 2011 Mar 31;6(3):e18373
Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism.
Endo Y, Beauchamp E, Woods D, Taylor WG, Toretsky JA, Uren A, Rubin JS
Molecular and cellular biology 2008 Apr;28(7):2368-79
Molecular and cellular biology 2008 Apr;28(7):2368-79
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Frizzled-3 in Mouse Embryo. Frizzled-3 was detected in immersion fixed frozen sections of mouse embryo (E11) using Mouse Frizzled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1001) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
