Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- BS-13239R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD3/Frizzled 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5 µg/mL) was used to stain the cell nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated ( bs-13239R ) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy)and used at 1:200 dilution for 40 minutes at 37°CDAPI(5 µg/mL) was used to stain the cell nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (bs-13239R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.