Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-51709 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SQCNLYMAAR KAVRKRPNQA LLENIALYLT HMLKIFGAVE EDSSLGFPVG GPGTSLSLEA TVMPYLQVLS EFREGVRKIA REQKVPEILQ LSDALRDNIL PELGVRFEDH EGLPTVVKLV DRNTLLKERE EKRRVE
- Concentration
- 0.1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARS in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells); Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla). Samples were probed using a CARS Polyclonal Antibody (Product # PA5-51709).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARS using CARS Polyclonal Antibody (Product # PA5-51709) (A) shows similar pattern to an independent CARS Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of CARS in human cell line U-2 OS shows positivity in cytoplasm. Samples were probed using a CARS Polyclonal Antibody (Product # PA5-51709).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human testis using CARS Polyclonal Antibody (Product # PA5-51709).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human cerebral cortex and skeletal muscle tissues using CARS Polyclonal Antibody (Product # PA5-51709). Corresponding CARS RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human cerebral cortex, pancreas, skeletal muscle and testis using CARS Polyclonal Antibody (Product # PA5-51709) (A) shows similar protein distribution across tissues to an independent CARS Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human skeletal muscle using CARS Polyclonal Antibody (Product # PA5-51709) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human cerebral cortex using CARS Polyclonal Antibody (Product # PA5-51709) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CARS in human pancreas using CARS Polyclonal Antibody (Product # PA5-51709).
