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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-31706 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CK2 beta Monoclonal Antibody (2F12F3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31706 has been tested in indirect ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F12F3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CK2 beta in human CSNK2B recombinant protein. Sample was incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CK2 beta in HEK293 (1) and CSNK2B cell lysate. Samples were incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CK2 beta in HeLa (1), Jurkat (2), K562 (3), HepG2 (4), C6 (5), SK-N-SH (6), NTERA-2 (7), MCF-7 (8), NIH/3T3 (9) cell lysate. Samples were incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Casein kinase II subunit beta was achieved by transfecting HeLa with Casein kinase II subunit beta specific siRNAs (Silencer® select Product # S3642, S3643). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Casein kinase II subunit beta knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with CK2 beta Monoclonal Antibody (2F12F3) (Product # MA5-31706, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Casein kinase II subunit beta.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CK2 beta Monoclonal Antibody (2F12F3) (Product # MA5-31706) and a ~25 kDa band corresponding to Casein kinase II subunit beta was observed along with few uncharacterised band (*) between ~35 kDa to ~110 kDa; across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), Jurkat (Lane 5), K-562 (Lane 6), THP-1 (Lane 7), NIH/3T3 (Lane 8), PC-12 (Lane 9), Mouse Testis (Lane 10) and Rat Testis (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of CK2 beta in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CK2 beta in MCF-7 cells (green). Sample was incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CK2 beta in paraffin-embedded cervical cancer tissue. Sample was incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CK2 beta in paraffin-embedded colon cancer tissue. Sample was incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CK2 beta in HeLa cells (green). Sample was incubated with CK2 beta monoclonal antibody (Product # MA5-31706) using a dilution of 1:200-1:400 followed by negative control (red).
