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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- PA5-23097 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: antigen standard for TLR1 (transient overexpression lysate), Ramos.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using a TLR1/CD281 polyclonal antibody (Product # PA5-23097) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR1 in Ramos, Raw, and TLR1 transfected cell lysate. Samples were incubated in TLR1 polyclonal antibody (Product # PA5-23097) followed by a goat anti-rabbit HRP secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TLR1 in human small intestine. Samples were incubated in TLR1 polyclonal antibody (Product # PA5-23097) using a dilution of 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface flow cytometric analysis of TLR1 in human PBMCs (monocyte-gated) using a TLR1/CD281 polyclonal antibody (Product # PA5-23097) at 0.5 µg/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1/CD281 polyclonal antibody (Product # PA5-23097). Goat anti-rabbit PE secondary antibody was used.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR1 in human PBMCs (lymphocyte-gated). Samples were incubated in TLR1 polyclonal antibody (Product # PA5-23097) using a dilution of 10 µl/10^6 cells. Shaded histogram represents cells alone, green represents isotype control that was used for this test. Antibody was conjugated to Alexa Fluor (R) 488.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR1 in human PBMCs (lymphocyte-gated). Samples were incubated in TLR1 polyclonal antibody (Product # PA5-23097) using a dilution of 10 µl/10^6 cells. Shaded histogram represents cells without antibody, green represents isotype control, red represents TLR1 antibody.
