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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 200-401-A68 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-A68, RRID:AB_2204162
- Product name
- Anti-TLR1 (RABBIT) Antibody - 200-401-A68
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-TLR1 Antibody. Lane 1: mouse spleen tissue whole cell lysate using 1.0 ug/ml. Lane 2: mouse spleen tissue whole cell lysate using 2.0 ug/ml. Lane 3: mouse spleen tissue whole cell lysate using 4.0 ug/ml. Load: 10 µg per lane. Primary antibody: TLR-1 antibody overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 90 kDa,~90 kDa for TLR 1. Other band(s): none.
- Validation comment
- Western Blot
