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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-00201 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00201, RRID:AB_1503433
- Product name
- Goat Polyclonal Hyaluronidase 1/HYAL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 2 (NP_695014.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1). Reported variants represent identical protein (NP_009296.1
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Neurofibromatosis-1 (Nf1) heterozygous brain microglia elaborate paracrine factors that promote Nf1-deficient astrocyte and glioma growth.
Daginakatte GC, Gutmann DH
Human molecular genetics 2007 May 1;16(9):1098-112
Human molecular genetics 2007 May 1;16(9):1098-112
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Hyaluronidase 1/HYAL1 Antibody [NBP1-00201] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
