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- Antibody Data
- Antigen structure
- References [18]
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- Immunohistochemistry [1]
- Flow cytometry [5]
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- Product number
- NB100-56536 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56536, RRID:AB_839012
- Product name
- Mouse Monoclonal TLR6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. TLR6 (86B1153.2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression of Toll-like receptors 2, 4 and 6 in the equine chorioallantois.
Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium.
Expression of Toll-like receptors in nasal epithelium in allergic rhinitis.
Naïve T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Identification of TLR10 as a key mediator of the inflammatory response to Listeria monocytogenes in intestinal epithelial cells and macrophages.
Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Toll-like receptor triggering in cord blood mesenchymal stem cells.
Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity.
Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet's disease etiopathogenesis.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Toll-like receptor 2-mediated human B cell differentiation.
Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas gingivalis with the Toll-like receptor 2-centred pattern recognition apparatus.
Evidence of Toll-like receptor molecules on human platelets.
Schöniger S, Gräfe H, Wipplinger M, Schoon HA
Veterinary immunology and immunopathology 2018 Dec;206:49-53
Veterinary immunology and immunopathology 2018 Dec;206:49-53
Expression of Toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium.
Schöniger S, Gräfe H, Schoon HA
Veterinary immunology and immunopathology 2017 Mar;185:7-13
Veterinary immunology and immunopathology 2017 Mar;185:7-13
Expression of Toll-like receptors in nasal epithelium in allergic rhinitis.
Renkonen J, Toppila-Salmi S, Joenväärä S, Mattila P, Parviainen V, Hagström J, Haglund C, Lehtonen M, Renkonen R
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Aug;123(8):716-25
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2015 Aug;123(8):716-25
Naïve T cells, unconventional NK and NKT cells, and highly responsive monocyte-derived macrophages characterize human cord blood.
López MC, Palmer BE, Lawrence DA
Immunobiology 2014 Oct;219(10):756-65
Immunobiology 2014 Oct;219(10):756-65
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Identification of TLR10 as a key mediator of the inflammatory response to Listeria monocytogenes in intestinal epithelial cells and macrophages.
Regan T, Nally K, Carmody R, Houston A, Shanahan F, Macsharry J, Brint E
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6084-92
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6084-92
Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands.
Oberg HH, Ly TT, Ussat S, Meyer T, Kabelitz D, Wesch D
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4733-40
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):4733-40
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Toll-like receptor triggering in cord blood mesenchymal stem cells.
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG, Netea MG, Latuhihin T, Bergevoet S, Raymakers RA, Kögler G, Figdor CC, Adema GJ, Torensma R
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity.
Peiser M, Koeck J, Kirschning CJ, Wittig B, Wanner R
Journal of leukocyte biology 2008 May;83(5):1118-27
Journal of leukocyte biology 2008 May;83(5):1118-27
Differential expression of toll-like receptor 6 on granulocytes and monocytes implicates the role of microorganisms in Behcet's disease etiopathogenesis.
Yavuz S, Elbir Y, Tulunay A, Eksioglu-Demiralp E, Direskeneli H
Rheumatology international 2008 Mar;28(5):401-6
Rheumatology international 2008 Mar;28(5):401-6
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Toll-like receptor 2-mediated human B cell differentiation.
Ganley-Leal LM, Liu X, Wetzler LM
Clinical immunology (Orlando, Fla.) 2006 Sep;120(3):272-84
Clinical immunology (Orlando, Fla.) 2006 Sep;120(3):272-84
Differential interactions of fimbriae and lipopolysaccharide from Porphyromonas gingivalis with the Toll-like receptor 2-centred pattern recognition apparatus.
Hajishengallis G, Tapping RI, Harokopakis E, Nishiyama S, Ratti P, Schifferle RE, Lyle EA, Triantafilou M, Triantafilou K, Yoshimura F
Cellular microbiology 2006 Oct;8(10):1557-70
Cellular microbiology 2006 Oct;8(10):1557-70
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR6 Antibody (86B1153.2) [NB100-56536] - Tonsil probed with TLR6 antibody at 5 ug/ml. Novus's human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR6 Antibody (86B1153.2) [NB100-56536] - Cell surface analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents anti-TLR6 antibody. goat anti-mouse IgG PE conjugated secondary antibody was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in human PBMC cells using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents PBMC without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the PE conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. 5 ug/10^6 cells. Shaded histogram represents Ramos cells without antibody; green represents isotype control (BD Pharmingen); red represents this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the FITC conjugate of NB100-56536. Staining of TLR6 in Ramos cells using this antibody. Green represents isotype control (mouse IgG1-FITC); red represents anti-TLR6 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR6 Antibody (86B1153.2) [NB100-56536] - Analysis using the FITC conjugate of NB100-56536. Staining of TLR6 in human lymphocytes using this antibody; red represents anti-TLR6 antibody.
