PAB28572

antibody from Abnova Corporation

Targeting: PSMD14 pad1, POH1, Rpn11

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28572

Provider

Abnova Corporation

Product name

PSMD14 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PSMD14.

Antigen sequence

AVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLML
GEFVDDYTVRVIDVFAMPQSGTGVSVEAVDPVFQA
KMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDIN
TQQSFEALSERAVAVVVDPIQSVKGKVVIDA


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: RT-4, lane 2: U-251 MG, lane 3: Liver and lane 4: Tonsil using PSMD14 polyclonal antibody (Cat # PAB28572).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of lane 1: NIH-3T3 and lane 2: NBT-II cell lysates using PSMD14 polyclonal antibody (Cat # PAB28572).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of U-2 OS cell line with PSMD14 polyclonal antibody (Cat # PAB28572) shows positivity in nucleus but not nucleoli and vesicles. Fixation/Permeabilization: PFA/Triton X-102

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human hippocampus with PSMD14 polyclonal antibody (Cat # PAB28572) shows strong nuclear positivity in neuronal cells. Retrieval method: HIER pH6

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)