Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00001846-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001846-D01P, RRID:AB_1573360
- Product name
- DUSP4 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human DUSP4 protein.
- Antigen sequence
MVTMEELREMDCSVLKRLMNRDENGGGAGGSGSHG
TLGLPSGGKCLLLDCRPFLAHSAGYILGSVNVRCN
TIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAV
IVYDERSPRAESLREDSTVSLVVQALRRNAERTDI
CLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSA
TEPLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYH
AARRDMLDALGITALLNVSSDCPNHFEGHYQYKCI
PVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHC
QAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRS
IISPNFSFMGQLLQFESQVLATSCAAEAASPSGPL
RERGKTPATPTSQFVFSFPVSVGVHSAPSSLPYLH
SPITTSPSC- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DUSP4 expression in transfected 293T cell line (H00001846-T01) by DUSP4 MaxPab polyclonal antibody.Lane 1: DUSP4 transfected lysate(43.00 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to DUSP4 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between DUSP4 and MAPK9. HeLa cells were stained with anti-DUSP4 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)