MA5-27814

antibody from Invitrogen Antibodies

Targeting: ARID5A MRF-1, RP11-363D14

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27814

Provider

Invitrogen Antibodies

Product name

ARID5A Monoclonal Antibody (GT1683)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 3xFlag human ARID5A-transfected 293T, THP-1, THP-1 nuclear extract

Antibody clone number

GT1683

Concentration

2.14 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ARID5A in THP-1 whole cell and nuclear extracts using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ARID5A monoclonal antibody (Product # MA5-27814) using a dilution of 1:500 followed by ECL substrate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ARID5A in non-transfected (-) and ARID5A -transfected (+) 293T whole cell extracts using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ARID5A monoclonal antibody (Product # MA5-27814) using a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814). THP-1 whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ARID5A Polyclonal Antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A Polyclonal Antibody ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) diluted by 1:10,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ARID5A was performed in paraffin-embedded mouse urinary bladder tissue using ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ARID5A was performed in paraffin-embedded mouse brain tissue using ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of ARID5A was performed in frozen-sectioned mouse mouse cerebellum tissue using ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) at a dilution of 1:250 (Green). Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of ARID5A was performed in THP-1 cells using ARID5A Monoclonal Antibody (GT1683) (Product # MA5-27814) (red) at a dilution of 1:50. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.