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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1445 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Prolactin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Prolactin in Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Prolactin is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_035294- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Completely humanizing prolactin rescues infertility in prolactin knockout mice and leads to human prolactin expression in extrapituitary mouse tissues.
Paternal recognition of adult offspring mediated by newly generated CNS neurons.
Blocking vascular endothelial growth factor-A inhibits the growth of pituitary adenomas and lowers serum prolactin level in a mouse model of multiple endocrine neoplasia type 1.
Christensen HR, Murawsky MK, Horseman ND, Willson TA, Gregerson KA
Endocrinology 2013 Dec;154(12):4777-89
Endocrinology 2013 Dec;154(12):4777-89
Paternal recognition of adult offspring mediated by newly generated CNS neurons.
Mak GK, Weiss S
Nature neuroscience 2010 Jun;13(6):753-8
Nature neuroscience 2010 Jun;13(6):753-8
Blocking vascular endothelial growth factor-A inhibits the growth of pituitary adenomas and lowers serum prolactin level in a mouse model of multiple endocrine neoplasia type 1.
Korsisaari N, Ross J, Wu X, Kowanetz M, Pal N, Hall L, Eastham-Anderson J, Forrest WF, Van Bruggen N, Peale FV, Ferrara N
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jan 1;14(1):249-58
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Jan 1;14(1):249-58
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Prolactin by Western Blot. Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Prolactin and Neutralization by Mouse Prolactin Antibody. Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Rec-ombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND50 is typically 0.25-1.0 µg/mL.
