H00005617-M02

antibody from Abnova Corporation

Targeting: PRL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005617-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005617-M02, RRID:AB_489709

Product name

PRL monoclonal antibody (M02), clone 1E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PRL.

Antigen sequence

LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSE
MFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKE
QAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGM
QEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPE
TKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCL
RRDSHKIDNYLKLLKCRIIHNNNC


Isotype

IgG

Antibody clone number

1E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PRL expression in transfected 293T cell line by PRL monoclonal antibody (M02), clone 1E1.Lane 1: PRL transfected lysate(25.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PRL is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol