PA5-120639

antibody from Invitrogen Antibodies

Targeting: PRMT8 HRMT1L3, HRMT1L4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120639

Provider

Invitrogen Antibodies

Product name

PRMT8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: 293T, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Cytoplasmic side, Lipid-anchor.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.85 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PRMT8 in normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells using a PRMT8 Polyclonal antibody (Product # PA5-120639) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PRMT8 in normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells using a PRMT8 Polyclonal antibody (Product # PA5-120639) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.