Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX116011 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116011, RRID:AB_2036782
- Product name
- DNMT1 antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in both Host and Viral Genomes.
Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone.
Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Antroquinonol D, isolated from Antrodia camphorata, with DNA demethylation and anticancer potential.
Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition.
The expression patterns and correlations of claudin-6, methy-CpG binding protein 2, DNA methyltransferase 1, histone deacetylase 1, acetyl-histone H3 and acetyl-histone H4 and their clinicopathological significance in breast invasive ductal carcinomas.
Vorinostat, SAHA, represses telomerase activity via epigenetic regulation of telomerase reverse transcriptase in non-small cell lung cancer cells.
Epigenetic mechanisms for silencing glutathione S-transferase m2 expression by hypermethylated specificity protein 1 binding in lung cancer.
Huang MH, Chou YW, Li MH, Shih TE, Lin SZ, Chuang HM, Chiou TW, Su HL, Harn HJ
Pharmacological research 2019 Jan;139:50-61
Pharmacological research 2019 Jan;139:50-61
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Weng YP, Hung PF, Ku WY, Chang CY, Wu BH, Wu MH, Yao JY, Yang JR, Lee CH
Oncotarget 2018 Jan 2;9(1):361-374
Oncotarget 2018 Jan 2;9(1):361-374
Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in both Host and Viral Genomes.
Chen YJ, Chen YL, Chang Y, Wu CC, Ko YC, Tsao SW, Chen JY, Lin SF
Journal of virology 2017 Aug 1;91(15)
Journal of virology 2017 Aug 1;91(15)
Secreted Protein Acidic and Rich in Cysteine (SPARC) Mediates Metastatic Dormancy of Prostate Cancer in Bone.
Sharma S, Xing F, Liu Y, Wu K, Said N, Pochampally R, Shiozawa Y, Lin HK, Balaji KC, Watabe K
The Journal of biological chemistry 2016 Sep 9;291(37):19351-63
The Journal of biological chemistry 2016 Sep 9;291(37):19351-63
Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
Hsieh YY, Huang TC, Lo HL, Jhan JY, Chen ST, Yang PM
Oncotarget 2016 May 10;7(19):27363-78
Oncotarget 2016 May 10;7(19):27363-78
DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
Li HK, Mai RT, Huang HD, Chou CH, Chang YA, Chang YW, You LR, Chen CM, Lee YH
Scientific reports 2016 Jun 27;6:28637
Scientific reports 2016 Jun 27;6:28637
The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Wijenayake S, Storey KB
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 2016 Apr;186(3):333-42
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 2016 Apr;186(3):333-42
Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Wen HC, Chuu CP, Chen CY, Shiah SG, Kung HJ, King KL, Su LC, Chang SC, Chang CH
PloS one 2015;10(4):e0125518
PloS one 2015;10(4):e0125518
Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells.
Aslanian A, Yates JR 3rd, Hunter T
DNA repair 2014 Mar;15:29-38
DNA repair 2014 Mar;15:29-38
Antroquinonol D, isolated from Antrodia camphorata, with DNA demethylation and anticancer potential.
Wang SC, Lee TH, Hsu CH, Chang YJ, Chang MS, Wang YC, Ho YS, Wen WC, Lin RK
Journal of agricultural and food chemistry 2014 Jun 18;62(24):5625-35
Journal of agricultural and food chemistry 2014 Jun 18;62(24):5625-35
Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition.
Lin YC, Lee YC, Li LH, Cheng CJ, Yang RB
Journal of cell science 2014 Jan 1;127(Pt 1):85-100
Journal of cell science 2014 Jan 1;127(Pt 1):85-100
The expression patterns and correlations of claudin-6, methy-CpG binding protein 2, DNA methyltransferase 1, histone deacetylase 1, acetyl-histone H3 and acetyl-histone H4 and their clinicopathological significance in breast invasive ductal carcinomas.
Xu X, Jin H, Liu Y, Liu L, Wu Q, Guo Y, Yu L, Liu Z, Zhang T, Zhang X, Dong X, Quan C
Diagnostic pathology 2012 Mar 29;7:33
Diagnostic pathology 2012 Mar 29;7:33
Vorinostat, SAHA, represses telomerase activity via epigenetic regulation of telomerase reverse transcriptase in non-small cell lung cancer cells.
Li CT, Hsiao YM, Wu TC, Lin YW, Yeh KT, Ko JL
Journal of cellular biochemistry 2011 Oct;112(10):3044-53
Journal of cellular biochemistry 2011 Oct;112(10):3044-53
Epigenetic mechanisms for silencing glutathione S-transferase m2 expression by hypermethylated specificity protein 1 binding in lung cancer.
Tang SC, Wu MF, Wong RH, Liu YF, Tang LC, Lai CH, Hsu CP, Ko JL
Cancer 2011 Jul 15;117(14):3209-21
Cancer 2011 Jul 15;117(14):3209-21
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Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE GTX116011 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT1 antibody [N1], N-term detects DNMT1 protein by western blot analysis.A. 30 ?g 293T whole cell extractB. 30 ?g A431 whole cell extractC. 30 ?g HeLa whole cell extractD. 30 ?g HepG2 whole cell extract5 % SDS-PAGEDNMT1 antibody [N1], N-term (GTX116011) dilution: 1:500
- Validation comment
- WB
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- DNMT1 antibody [N1], N-term detects DNMT1 protein by western blot analysis.A. 30 ?g Jurkat whole cell extractB. 30 ?g Raji whole cell extractC. 30 ?g NCI-H929 whole cell extract5 % SDS-PAGEDNMT1 antibody [N1], N-term (GTX116011) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT1 antibody [N1], N-term detects DNMT1 protein by western blot analysis.A. 30 ?g 293T whole cell extractB. 30 ?g A431 whole cell extractC. 30 ?g HeLa whole cell extractD. 30 ?g HepG2 whole cell extract5% SDS-PAGEDNMT1 antibody [N1], N-term (GTX116011) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and DNMT1 knockout (KO) 293T cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using DNMT1(GTX116011) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT1 antibody [N1], N-term detects DNMT1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNMT1 protein stained by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:2000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Supportive validation
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- Main image
- Experimental details
- DNMT1 antibody [N1], N-term immunoprecipitates DNMT1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of DNMT1 protein by 4 £gg DNMT1 antibody [N1], N-term (GTX116011)5 % SDS-PAGEThe immunoprecipitated DNMT1 protein was detected by DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT1 antibody [N1], N-term detects DNMT1 protein at cytoplasm and nucleus in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. DNMT1 antibody [N1], N-term (GTX116011) diluted at 1:500.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HCT116 chromatin extract and 5 £gg of either control rabbit IgG or anti-DNMT1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ILIR2.