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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX30364 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX30364, RRID:AB_385506
- Product name
- DNMT1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
Ratovitski EA
Cell cycle (Georgetown, Tex.) 2014;13(5):749-61
Cell cycle (Georgetown, Tex.) 2014;13(5):749-61
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR
Nature genetics 2003 Jan;33(1):61-5
Nature genetics 2003 Jan;33(1):61-5
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
Beaulieu N, Morin S, Chute IC, Robert MF, Nguyen H, MacLeod AR
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot: Dnmt1 Antibody [GTX30364] - Analysis using GTX30364 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 μgs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 μgs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Dnmt1 Antibody [GTX30364] - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Dnmt1 Antibody [GTX30364] - IHC staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Dnmt1 Antibody [GTX30364] - IHC staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.
