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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001786-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001786-M01, RRID:AB_565677
- Product name
- DNMT1 monoclonal antibody (M01), clone 2B5
- Antibody type
- Monoclonal
- Antigen
- DNMT1 (NP_001370, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDS
LTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRK
EELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNG
RLENG- Isotype
- IgG
- Vial size
- 50 μg
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Dynamic regulation of DNA methyltransferases in human oocytes and preimplantation embryos after assisted reproductive technologies.
Petrussa L, Van de Velde H, De Rycke M
Molecular human reproduction 2014 Sep;20(9):861-74
Molecular human reproduction 2014 Sep;20(9):861-74
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DNMT1 monoclonal antibody (M01), clone 2B5. Western Blot analysis of DNMT1 expression in MCF-7.
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DNMT1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to DNMT1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DNMT1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
