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- Antibody Data
- Antigen structure
- References [27]
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- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- NB100-264 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-264, RRID:AB_10000772
- Product name
- Rabbit Polyclonal DNMT1 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Prenatal bisphenol-A exposure altered exploratory and anxiety-like behaviour and induced non-monotonic, sex-specific changes in the cortical expression of CYP19A1, BDNF and intracellular signaling proteins in F1 rats.
lncRNA HOTAIRM1 promotes osteogenesis of hDFSCs by epigenetically regulating HOXA2 via DNMT1 in vitro.
Dnmt1 is required for proximal-distal patterning of the lung endoderm and for restraining alveolar type 2 cell fate.
DNMT1 regulates expression of MHC class I in post-mitotic neurons.
The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases.
Multiple structural and epigenetic defects in the human leukocyte antigen class I antigen presentation pathway in a recurrent metastatic melanoma following immunotherapy.
Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.
Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells.
Mitochondrial DNMT3A and DNA methylation in skeletal muscle and CNS of transgenic mouse models of ALS.
Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies.
DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation.
Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases.
Insufficient maintenance DNA methylation is associated with abnormal embryonic development.
Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma.
DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation.
Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL.
The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression.
Opposing roles of Dnmt1 in early- and late-stage murine prostate cancer.
Remote sensing alone is insufficient for quantifying changes in forest cover.
Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model.
Epigenetic regulation of X-linked cancer/germline antigen genes by DNMT1 and DNMT3b.
Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication.
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells.
Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells.
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Raja GL, Lite C, Subhashree KD, Santosh W, Barathi S
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Aug;142:111442
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Aug;142:111442
lncRNA HOTAIRM1 promotes osteogenesis of hDFSCs by epigenetically regulating HOXA2 via DNMT1 in vitro.
Chen Z, Zheng J, Hong H, Chen D, Deng L, Zhang X, Ling J, Wu L
Journal of cellular physiology 2020 Apr 23;
Journal of cellular physiology 2020 Apr 23;
Dnmt1 is required for proximal-distal patterning of the lung endoderm and for restraining alveolar type 2 cell fate.
Liberti DC, Zepp JA, Bartoni CA, Liberti KH, Zhou S, Lu M, Morley MP, Morrisey EE
Developmental biology 2019 Oct 15;454(2):108-117
Developmental biology 2019 Oct 15;454(2):108-117
DNMT1 regulates expression of MHC class I in post-mitotic neurons.
Gustafsson JR, Katsioudi G, Degn M, Ejlerskov P, Issazadeh-Navikas S, Kornum BR
Molecular brain 2018 Jul 3;11(1):36
Molecular brain 2018 Jul 3;11(1):36
The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases.
Gu H, Gao J, Guo W, Zhou Y, Kong Q
Molecular medicine reports 2017 Jul;16(1):591-596
Molecular medicine reports 2017 Jul;16(1):591-596
Multiple structural and epigenetic defects in the human leukocyte antigen class I antigen presentation pathway in a recurrent metastatic melanoma following immunotherapy.
Chang CC, Pirozzi G, Wen SH, Chung IH, Chiu BL, Errico S, Luongo M, Lombardi ML, Ferrone S
The Journal of biological chemistry 2015 Oct 30;290(44):26562-75
The Journal of biological chemistry 2015 Oct 30;290(44):26562-75
Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.
Cui Y, Irudayaraj J
Nucleic acids research 2015 Mar 31;43(6):3046-55
Nucleic acids research 2015 Mar 31;43(6):3046-55
Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells.
Vispé S, Deroide A, Davoine E, Desjobert C, Lestienne F, Fournier L, Novosad N, Bréand S, Besse J, Busato F, Tost J, De Vries L, Cussac D, Riond J, Arimondo PB
Oncotarget 2015 Jun 20;6(17):15265-82
Oncotarget 2015 Jun 20;6(17):15265-82
Mitochondrial DNMT3A and DNA methylation in skeletal muscle and CNS of transgenic mouse models of ALS.
Wong M, Gertz B, Chestnut BA, Martin LJ
Frontiers in cellular neuroscience 2013;7:279
Frontiers in cellular neuroscience 2013;7:279
Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies.
Gros C, Chauvigné L, Poulet A, Menon Y, Ausseil F, Dufau I, Arimondo PB
Nucleic acids research 2013 Oct;41(19):e185
Nucleic acids research 2013 Oct;41(19):e185
DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation.
Lai AY, Mav D, Shah R, Grimm SA, Phadke D, Hatzi K, Melnick A, Geigerman C, Sobol SE, Jaye DL, Wade PA
Genome research 2013 Dec;23(12):2030-41
Genome research 2013 Dec;23(12):2030-41
Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases.
Selvakumar T, Gjidoda A, Hovde SL, Henry RW
The Journal of biological chemistry 2012 Mar 2;287(10):7039-50
The Journal of biological chemistry 2012 Mar 2;287(10):7039-50
Insufficient maintenance DNA methylation is associated with abnormal embryonic development.
Yin LJ, Zhang Y, Lv PP, He WH, Wu YT, Liu AX, Ding GL, Dong MY, Qu F, Xu CM, Zhu XM, Huang HF
BMC medicine 2012 Mar 13;10:26
BMC medicine 2012 Mar 13;10:26
Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma.
Chang VH, Chu PY, Peng SL, Mao TL, Shan YS, Hsu CF, Lin CY, Tsai KK, Yu WC, Ch'ang HJ
The American journal of pathology 2012 Aug;181(2):423-30
The American journal of pathology 2012 Aug;181(2):423-30
DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation.
Shaknovich R, Cerchietti L, Tsikitas L, Kormaksson M, De S, Figueroa ME, Ballon G, Yang SN, Weinhold N, Reimers M, Clozel T, Luttrop K, Ekstrom TJ, Frank J, Vasanthakumar A, Godley LA, Michor F, Elemento O, Melnick A
Blood 2011 Sep 29;118(13):3559-69
Blood 2011 Sep 29;118(13):3559-69
Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL.
Kaminskyy VO, Surova OV, Vaculova A, Zhivotovsky B
Carcinogenesis 2011 Oct;32(10):1450-8
Carcinogenesis 2011 Oct;32(10):1450-8
The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression.
Al-Salihi M, Yu M, Burnett DM, Alexander A, Samlowski WE, Fitzpatrick FA
Epigenetics 2011 Aug;6(8):1021-8
Epigenetics 2011 Aug;6(8):1021-8
Opposing roles of Dnmt1 in early- and late-stage murine prostate cancer.
Kinney SR, Moser MT, Pascual M, Greally JM, Foster BA, Karpf AR
Molecular and cellular biology 2010 Sep;30(17):4159-74
Molecular and cellular biology 2010 Sep;30(17):4159-74
Remote sensing alone is insufficient for quantifying changes in forest cover.
Reams GA, Brewer CK, Guldin RW
Proceedings of the National Academy of Sciences of the United States of America 2010 Sep 21;107(38):E145; author reply E146
Proceedings of the National Academy of Sciences of the United States of America 2010 Sep 21;107(38):E145; author reply E146
Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model.
Morey Kinney SR, Smiraglia DJ, James SR, Moser MT, Foster BA, Karpf AR
Molecular cancer research : MCR 2008 Aug;6(8):1365-74
Molecular cancer research : MCR 2008 Aug;6(8):1365-74
Epigenetic regulation of X-linked cancer/germline antigen genes by DNMT1 and DNMT3b.
James SR, Link PA, Karpf AR
Oncogene 2006 Nov 2;25(52):6975-85
Oncogene 2006 Nov 2;25(52):6975-85
Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication.
Estève PO, Chin HG, Smallwood A, Feehery GR, Gangisetty O, Karpf AR, Carey MF, Pradhan S
Genes & development 2006 Nov 15;20(22):3089-103
Genes & development 2006 Nov 15;20(22):3089-103
DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.
Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR
Nature genetics 2003 Jan;33(1):61-5
Nature genetics 2003 Jan;33(1):61-5
Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells.
Qiu YY, Mirkin BL, Dwivedi RS
Cancer detection and prevention 2002;26(6):444-53
Cancer detection and prevention 2002;26(6):444-53
Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells.
Qiu YY, Mirkin BL, Dwivedi RS
Cancer detection and prevention 2002;26(6):444-53
Cancer detection and prevention 2002;26(6):444-53
An essential role for DNA methyltransferase DNMT3B in cancer cell survival.
Beaulieu N, Morin S, Chute IC, Robert MF, Nguyen H, MacLeod AR
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Rhee I, Bachman KE, Park BH, Jair KW, Yen RW, Schuebel KE, Cui H, Feinberg AP, Lengauer C, Kinzler KW, Baylin SB, Vogelstein B
Nature 2002 Apr 4;416(6880):552-6
Nature 2002 Apr 4;416(6880):552-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DNMT1 Antibody [NB100-264] - Image shows a specific band for Dnmt1 in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-264] - Analysis using NB100-264 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ug of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ug of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-264] - DNMT1 in Fibroblasts. 30 ug of whole cell lysate. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT1 Antibody [NB100-264] - Impact of extensive DNMT1 depletion in HCT116 cells on TP73 promoter methylation HCT116 human colon cancer cells have been transfected every two days for four days with the same DNMT1 siRNA (1(6)) used in KG1 cells, or exposed for 72 hours to a dose range of DAC (0, 0.001, 0.01, 0.03, 0.1, 1, 10 uM). (a) Two days after the first transfection, total proteins were extracted and subjected to Western blotting with DNMT1 and DNMT3A antibodies. Actin was used as loading control. The percentages of residual DNMT protein levels are indicated relative to the corresponding untreated controls normalized to 100%. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/3317/text/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.
