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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- PA5-20944 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is Hela cell lysate.
- Concentration
- 1 mg/mL
Submitted references A Zic2-regulated switch in a noncanonical Wnt/βcatenin pathway is essential for the formation of bilateral circuits.
Morenilla-Palao C, López-Cascales MT, López-Atalaya JP, Baeza D, Calvo-Díaz L, Barco A, Herrera E
Science advances 2020 Nov;6(46)
Science advances 2020 Nov;6(46)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HeLa cell lysate using a APC2 polyclonal antibody (Product # PA5-20944) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of APC2 in HeLa cell lysate with APC2 Polyclonal Antibody (Product # PA5-20944) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of APC2 in HeLa cells with APC2 Polyclonal Antibody (Product # PA5-20944) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of APC2 in HeLa cells with APC2 Polyclonal Antibody (Product # PA5-20944) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Zic2 activates a program that leads to the accumulation of betacatenin in RGC axons. ( A ) In situ hybridization for Fzd8 in a coronal section of an E16.5 mouse retina. Bottom panel shows higher magnification of the squared area. ( B ) Immunofluorescence for Apc2 and betacatenin in the growth cone of axons growing from explants electroporated with EGFP- or Zic2-encoding plasmids. Scale bar, 10 mum. ( C ) Quantification of immunofluorescence intensity for Apc2 (upper graph) and betacatenin (lower graph) (** P < 0.01 and *** P < 0.001) ( n = mean of at least four growth cones/explant). ( D ) RFP fluorescence in whole-mount E16.5 retinas electroporated at E13.5 with the indicated plasmids. Panels at the right corner show targeted/EGFP cells in the same whole-mounted retinas. Scale bar, 100 mum. ( E ) Normalized quantification of RFP fluorescence intensity in whole-mount electroporated retinas. n = number of retinas (unpaired t test with Welch's correction, ** P < 0.001). ( F ) Optic chiasms from E16.5 embryos electroporated with plasmids encoding for betacatenin or Delta90-betaCatenin-DeltaCT. Scale bar, 100 mum. ( G ) Percentage of contralaterally projecting axons at the optic chiasm normalized to the total number of targeted axons ( n = number of embryos) (two-tailed unpaired t test, *** P < 0.001). Results show means +- SEM. All results come from at least three independent experiments.
