Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [1]
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Validation data
Reference
Comment
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- Product number
- H00000324-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000324-D01P, RRID:AB_1672972
- Product name
- APC purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human APC protein.
- Antigen sequence
MAAASYDQLLKQVEALKMENSNLRQELEDNSNHLT
KLETEASNMKEVLKQLQGSIEDEAMASSGQIDLLE
RLKELNLDSSNFPGVKLRSKMSLRSYGSREGSVSS
RSGECSPVPMGSFPRRGFVNGSRESTGYLEELEKE
RSLLLADLDKEEKEKDWYYAQLQNLTKRIDSLPLT
ENFSLQTDMTRRQLEYEARQIRVAMEEQLGTCQDM
EKRAQRRIARIQQIEKDILRIRQLLQSQATEAERS
SQNKHETGSHDAERQNEGQGVGEINMATSGNGQGS
TTRMDHETASVLSSSSTHSAPRRLTSHLGTKVEMV
YSLLSMLGTHDKDDMSRTLLAMSSSQDSCISMRQS
GCLPLLIQLLHGNDKDSVLLGNSRGSKEARARASA
ALHNIIHSQPDDKRGRREIRVLHLLEQIRAYCETC
WEWQEAHEPGMDQDKNPMPAPVEHQICPAVCVLMK
LSFDEEHRHAMNELGGLQAIAELLQVDCEMYGLTN
DHYSITLRRYAGMALTNLTFGDVANKATLCSMKGC
MRALVAQLKSESEDLQQVIASVLRNLSWRADVNSK
KTLREVGSVKALMECALEVKKESTLKSVLSALWNL
SAHCTENKADICAVDGALAFLVGTLTYRSQTNTLA
IIESGGGILRNVSSLIATNEDHRQILRENNCLQTL
LQHLKSHSLTIVSNACGTLWNLSARNPKDQEALWD
MGAVSMLKNLIHSKHKMIAMGSAAALRNLMANRPA
KYKDANIMSPGSSLPSLHVRKQKALEAELDAQHLS
ETFDNIDNLSPKASHRSKQRHKQSLYGDYVFDTNR
HDDNRSDNFNTGNMTVLSPYLNTTVLPSSSSSRGS
LDSSRSEKDRSLERERGIGLGNYHPATENPGTSSK
RGLQISTTAAQIAKVMEEVSAIHTSQEDRSSGSTT
ELHCVTDERNALRRSSAAHTHSNTYNFTKSENSNR
TCSMPYAKLEYKRSSNDSLNSVSSSDGYGKRGQMK
PSIESYSEDDESKFCSYGQYPADLAHKIHSANHMD
DNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWA
RPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDK
HLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHG
INQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEE
EERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSS
QKQSFSFSKSSSGQSSKTEHMSSSSENTSTPSSNA
KRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQ
TYCVEDTPICFSRCSSLSSLSSAEDEIGCNQTTQE
ADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHP
RTKSSRLQGSSLSSESARHKAVEFSSGAKSPSKSG
AQTPKSPPEHYVQETPLMFSRCTSVSSLDSFESRS
IASSVQSEPCSGMVSGIISPSDLPDSPGQTMPPSR
SKTPPPPPQTAQTKREVPKNKAPTAEKRESGPKQA
AVNAAVQRVQVLPDADTLLHFATESTPDGFSCSSS
LSALSLDEPFIQKDVELRIMPPVQENDNGNETESE
QPKESNENQEKEAEKTIDSEKDLLDDSDDDDIEIL
EECIISAMPTKSSRKAKKPAQTASKLPPPVARKPS
QLPVYKLLPSQNRLQPQKHVSFTPGDDMPRVYCVE
GTPINFSTATSLSDLTIESPPNELAAGEGVRGGAQ
SGEFEKRDTIPTEGRSTDEAQGGKTSSVTIPELDD
NKAEEGDILAECINSAMPKGKSHKPFRVKKIMDQV
QQASASSSAPNKNQLDGKKKKPTSPVKPIPQNTEY
RTRVRKNADSKNNLNAERVFSDNKDSKKQNLKNNS
KVFNDKLPNNEDRVRGSFAFDSPHHYTPIEGTPYC
FSRNDSLSSLDFDDDDVDLSREKAELRKAKENKES
EAKVTSHTELTSNQQSANKTQAIAKQPINRGQPKP
ILQKQSTFPQSSKDIPDRGAATDEKLQNFAIENTP
VCFSHNSSLSSLSDIDQENNNKENEPIKETEPPDS
QGEPSKPQASGYAPKSFHVEDTPVCFSRNSSLSSL
SIDSEDDLLQECISSAMPKKKKPSRLKGDNEKHSP
RNMGGILGEDLTLDLKDIQRPDSEHGLSPDSENFD
WKAIQEGANSIVSSLHQAAAAACLSRQASSDSDSI
LSLKSGISLGSPFHLTPDQEEKPFTSNKGPRILKP
GEKSTLETKKIESESKGIKGGKKVYKSLITGKVRS
NSEISGQMKQPLQANMPSISRGRTMIHIPGVRNSS
SSTSPVSKKGPPLKTPASKSPSEGQTATTSPRGAK
PSVKSELSPVARQTSQIGGSSKAPSRSGSRDSTPS
RPAQQPLSRPIQSPGRNSISPGRNGISPPNKLSQL
PRTSSPSTASTKSSGSGKMSYTSPGRQMSQQNLTK
QTGLSKNASSIPRSESASKGLNQMNNGNGANKKVE
LSRMSSTKSSGSESDRSERPVLVRQSTFIKEAPSP
TLRRKLEESASFESLSPSSRPASPTRSQAQTPVLS
PSLPDMSLSTHSSVQAGGWRKLPPNLSPTIEYNDG
RPAKRHDIARSHSESPSRLPINRSGTWKREHSKHS
SSLPRVSTWRRTGSSSSILSASSESSEKAKSEDEK
HVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNS
TSQTVSSGATNGAESKTLIYQMAPAVSKTEDVWVR
IEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIK
DSKDNQAKQNVGNGSVPMRTVGLENRLNSFIQVDA
PDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSS
SSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSA
RPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHS
GSYLVTSV- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of APC expression in transfected 293T cell line (H00000324-T02) by APC MaxPab polyclonal antibody.Lane 1: APC transfected lysate(312.73 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between APC and CTNNB1. HeLa cells were stained with anti-APC rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)