693702

antibody from BioLegend

Targeting: CCND2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

693702

Provider

BioLegend

Proper citation

BioLegend Cat#693702, RRID:AB_2650677

Product name

Purified anti-Cyclin D2

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W15213A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-Cyclin D2 (clone W15213A) antibody and competitor’s clone at the concentration suggested by the manufacturer. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.

Conjugate

Unconjugated